منابع مشابه
Southern Blotting and Related DNA Detection Techniques
Southern blotting is one of the central techniques in molecular biology. First devised by E.M. Southern (1975), Southern blotting results in transfer of DNA molecules, usually restriction fragments, from an electrophoresis gel to a nitrocellulose or nylon sheet (referred to as a ‘membrane’), in such a way that the DNA banding pattern present in the gel is reproduced on the membrane. During tran...
متن کاملWestern Blotting
Histological Analysis and Immunohistochemistry Renal sections (3 μm thick) were stained with periodic acid-Schiff (PAS) and examined under light microscopy. Glomerulosclerosis index was semiquantitatively graded as 0 to 4+; vascular injury score as 0 to 3+; and tubulointerstitial injury score as 0 to 5+, according to the criteria reported previously. Ultrastructure of glomerular podocytes was a...
متن کاملDiagnosis of cutaneous tuberculosis in biopsy specimens by PCR and southern blotting.
AIMS To evaluate the use of a gene amplification and hybridisation method for detecting mycobacterial nucleic acid as a possible diagnostic method for cutaneous tuberculosis infection. METHODS Biopsy specimens from 20 patients with various skin conditions of possible tuberculous aetiology were studied. Six patients had ulcerative nodules, seven lupiform lesions, two non-necrotic granulomas, o...
متن کاملModified agarose gel for high-performance Southern blotting of short DNA fragments.
Southern blotting is a common method used for the study of gene organization. Current methods of DNA transfer for Southern blotting, however, can be inefficient for high concentration agarose gels. Here, we report a method for high-performance Southern blotting of short DNA fragments such as nucleosomal DNAs by using a discontinuous agarose zone gel. The results show that sharp and well-resolve...
متن کاملPCR, Southern blotting and oocyte expression to assess the efficacy of subtractive hybridization.
Subtractive hybridization is a technique designed to isolate genes and transcripts abundant in or unique to a particular condition. The method has been utilized for constructing a subtracted cDNA library, differential cloning and identifying differentially expressed genes in specific cell lines (3–6,8,9). The analysis of the transcripts isolated by using this technology can be most difficult, i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the Nutrition Society
سال: 1996
ISSN: 0029-6651,1475-2719
DOI: 10.1079/pns19960052